General lab safety and aseptic technique
Making, running, staining agarose gels
Preparation of chemically-competent E.coli
Heat shock transformation of chemically-competent E.coli
Preparation of electrocompetent cells of Pseudomonas or E.coli, and electroporation
Preparation of electrocompetent cells of Mycobacterium, and electroporation
Genomic DNA extraction with CTAB
Genomic DNA extraction with bead beater
Resting cell experiments with bacteria
Plasmid miniprep - 5 ml - spin column
Plasmid midiprep - 50 ml - spin column
Plasmid midiprep - 50 ml - phenol-chloroform
Polymerase chain reaction (PCR) and primer design
Purification of PCRs or digests via spin column
Purification of PCRs or digests from gel by spin column
Quantitation and digestion of plasmid DNA
Cloning into plasmids - digestion, ligation, troubleshooting
Screening recombinant clones (patch, PCR, digest)