General lab safety and aseptic technique
Making and running agarose gels
Preparation of chemically competent cells of E.coli
Preparation of electrocompetent cells of E.coli or Pseudomonas
Electroporation of E.coli and Pseudomonas
Transformation of competent cells of E.coli by heat shock
Genomic DNA prep – CTAB method
Genomic DNA prep – beadbeater method
Resting cell experiments with bacteria
Gas chromatography of headspace samples
Plasmid prep – column method – 5 ml culture
Plasmid prep – column method – 50 ml culture
Plasmid prep – phenol method – 50 ml culture
Purification of PCRs or digests via spin column
Purification of PCRs or digests from gel via spin column
Cloning DNA – digestion and ligation